期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 106, 期 32, 页码 13517-13522出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0906413106
关键词
deadenylation-dependent mRNA decay; HCV; host factors; LSm1-7; Rck/p54
资金
- Spanish Ministerio de Educacion y Ciencia [BFU2004-00654, BFU2007-66933/BMC]
- Deutsche Forschungsgemeinschaft [Me 1061/4]
Inevitably, viruses depend on host factors for their multiplication. Here, we show that hepatitis C virus (HCV) RNA translation and replication depends on Rck/p54, LSm1, and PatL1, which regulate the fate of cellular mRNAs from translation to degradation in the 5'-3'-deadenylation-dependent mRNA decay pathway. The requirement of these proteins for efficient HCV RNA translation was linked to the 5' and 3' untranslated regions (UTRs) of the viral genome. Furthermore, LSm1-7 complexes specifically interacted with essential cis-acting HCV RNA elements located in the UTRs. These results bridge HCV life cycle requirements and highly conserved host proteins of cellular mRNA decay. The previously described role of these proteins in the replication of 2 other positive-strand RNA viruses, the plant brome mosaic virus and the bacteriophage Q beta, pinpoint a weak spot that may be exploited to generate broad-spectrum antiviral drugs.
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