期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 106, 期 9, 页码 3496-3501出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0809269106
关键词
astrocytes; Bergmann glia; in vivo; 2-photon microscopy; G-CaMP2
资金
- NINDS NIH HHS [NS045193, R01 NS045193] Funding Source: Medline
Multicellular glial calcium waves may locally regulate neural activity or brain energetics. Here, we report a diffusion- driven astrocytic signal in the normal, intact brain that spans many astrocytic processes in a confined volume without fully encompassing any one cell. By using 2-photon microscopy in rodent cerebellar cortex labeled with fluorescent indicator dyes or the calcium-sensor protein G-CaMP2, we discovered spontaneous calcium waves that filled approximately ellipsoidal domains of Bergmann glia processes. Waves spread in 3 dimensions at a speed of 4-11 mu m/s to a diameter of approximate to 50 mu m, slowed during expansion, and were reversibly blocked by P2 receptor antagonists. Consistent with the hypothesis that ATP acts as a diffusible trigger of calcium release waves, local ejection of ATP triggered P2 receptor-mediated waves that were refractory to repeated activation. Transglial waves represent a means for purinergic signals to act with local specificity to modulate activity or energetics in local neural circuits.
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