期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 106, 期 10, 页码 3829-3834出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0808382106
关键词
brachyury; chromatin immunopreciptitation; microarray
资金
- Medical Research Council [G0700213] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- Medical Research Council [G0300723B, G0700213] Funding Source: researchfish
- MRC [G0700213] Funding Source: UKRI
Using chromatin immunoprecipitation combined with genomic microarrays we have identified targets of No tail (Ntl), a zebrafish Brachyury ortholog that plays a central role in mesoderm formation. We show that Ntl regulates a downstream network of other transcription factors and identify an in vivo Ntl binding site that resembles the consensus T-box binding site (TBS) previously identified by in vitro studies. We show that the notochord-expressed gene floating head (flh) is a direct transcriptional target of Ntl and that a combination of TBSs in the flh upstream region are required for Ntl-directed expression. Using our genome-scale data we have assembled a preliminary gene regulatory network that begins to describe mesoderm formation and patterning in the early zebrafish embryo.
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