期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 37, 页码 13730-13735出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0806100105
关键词
erythrocyte; febrile temperature; malaria; mechanical properties; optical techniques
资金
- National Institutes of Health [P41-RR02594-18, 1-R01-GM076689-01]
- National Science Foundation [DBI-0754339]
- Interdisciplinary Research Group
- Singapore-MIT Alliance
- Cambridge Foundation Fellowship
- Samsung Scholarship
- Whitaker Health Science Fellowship
- Global Enterprise for MicroMechanics and Molecular Medicine (GEM4)
Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in A falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.
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