期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 46, 页码 17670-17675出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0805130105
关键词
drug discovery; immunoglobulins; streptavidin; selection; affinity chromatography
资金
- Eidgenossische Technische Hochschule
- Philochem AG
- Kommission for Technologie und Innovation [8868.1]
- Gebert-Ruf Foundation [GRS-076/06]
- Swiss National Science Foundation
- European Union [STROMA, IMMUNO-PDT]
DNA encoding facilitates the construction and screening of large chemical libraries. Here, we describe general strategies for the stepwise coupling of coding DNA fragments to nascent organic molecules throughout individual reaction steps as well as the first implementation of high-throughput sequencing for the identification and relative quantification of the library members. The methodology was exemplified in the construction of a DNA-encoded chemical library containing 4,000 compounds and in the discovery of binders to streptavidin, matrix metalloproteinase 3, and polyclonal human IgG.
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