期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 34, 页码 12313-12318出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0805709105
关键词
algal viruses; dual targeting; K plus channel sorting; PBCV-1; Esv-1
资金
- Deutsche Forschungsgemeinschaft [GRK340, SPP11131]
- Public Health Service [GM32441]
- National Institutes of Health (NIH) [P21RR15635]
- National Center for Research Resources
- McKnight Foundation for Neuroscience
- NIH
- Ministero dell' Universita e della Ricerca Scientifica
K+ channels operate in the plasma membrane and in membranes of organelles including mitochondria. The mechanisms and topogenic information for their differential synthesis and targeting is unknown. This article describes 2 similar viral K+ channels that are differentially sorted; one protein (Kesv) is imported by the Tom complex into the mitochondria, the other (Kcv) to the plasma membrane. By creating chimeras we discovered that mitochondrial sorting of Kesv depends on a hierarchical combination of N- and C-terminal signals. Crucial is the length of the second transmembrane domain; extending its C terminus by >= 2 hydrophobic amino acids redirects Kesv from the mitochondrial to the plasma membrane. Activity of Kesv in the plasma membrane is detected electrically or by yeast rescue assays only after this shift in sorting. Hence only minor structural alterations in a transmembrane domain are sufficient to switch sorting of a K+ channel between the plasma membrane and mitochondria.
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