期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 13, 页码 5195-5200出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0712350105
关键词
CD5; Mac-1; B cell development; neonatal; IgM
资金
- NIAID NIH HHS [R01 AI076434, AI 076434, R56 AI076434] Funding Source: Medline
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R56AI076434, R01AI076434] Funding Source: NIH RePORTER
Peritoneal cavity (PerC) B-1 cells have long been known to express CD11b, which is coexpressed with CD18 to form the Mac-1/CR3 complement receptor and adhesion molecule. However, although all PerC B-1 cells are commonly believed to express CD11b, we show here that nearly half of the cells in each of the PerC B-1 subsets (B-1a and B-1b) do not express this surface receptor. The CD11b(+) cells in each B-1 subset are larger and more granular and express higher levels of surface IgM than the CD11b-B-1 cells. In addition, the CD11b(+) B-1 cells initiate the formation of tightly associated doublets that are present at high frequency in adult PerC. Finally, and most importantly from a developmental stand-point, the CD11b(+) B-1 cells have a limited reconstitution capability: when sorted and transferred into congenic recipients, they reconstitute their own (CD11b(+)) B-1 subset but do not reconstitute the CD11b(-) B-1 subset. In contrast, CD11b(-) B-1 cells transferred under the same conditions efficiently replenish all components of the PerC B-1 population in appropriate proportions. During ontogeny, CD11b- B-1 cells appear before CD11b(+) B-1 cells. However, the clear phenotypic differences between the neonatal and adult CD11b B-1 subsets argue that although CD11b(-) B-1 give rise to CD11b(+) B-1 in both cases different forces may regulate this transition.
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