期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 48, 页码 18770-18775出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0800793105
关键词
deafness; mouse models; P2x7 receptor; pannexin; biosensor cells
资金
- Fondazione Cariparo/Progetti di Eccellenza 2006
- Telethon Italy [GGP05131]
- European Commission [LSHGCT20054512063]
- National Institutes of Health/National Institute on Deafness and Other Communication Disorders [DC007630]
- Medical Research Council [G0501173, G9708005] Funding Source: researchfish
Extracellular ATP controls various signaling systems including propagation of intercellular Ca2+ signals (ICS). Connexin hemichannels, P2x7 receptors (P2x7Rs), pannexin channels, anion channels, vesicles, and transporters are putative conduits for ATP release, but their involvement in ICS remains controversial. We investigated ICS in cochlear organotypic cultures, in which ATP acts as an IP3-generating agonist and evokes Ca2+ responses that have been linked to noise-induced hearing loss and development of hair cell-afferent synapses. Focal delivery of ATP or photostimulation with caged IP3 elicited Ca2+ responses that spread radially to several orders of unstimulated cells. Furthermore, we recorded robust Ca2+ signals from an ATP biosensor apposed to supporting cells outside the photostimulated area in WT cultures. ICS propagated normally in cultures lacking either P2x7R or pannexin-1 (Px1), as well as in WT cultures exposed to blockers of anion channels. By contrast, Ca2+ responses failed to propagate in cultures with defective expression of connexin 26 (Cx26) or Cx30. A companion paper demonstrates that, if expression of either Cx26 or Cx30 is blocked, expression of the other is markedly down-regulated in the outer sulcus. Lanthanum, a connexin hemichannel blocker that does not affect gap junction (GJ) channels when applied extracellularly, limited the propagation of Ca2+ responses to cells adjacent to the photostimulated area. Our results demonstrate that these connexins play a dual crucial role in inner ear Ca2+ signaling: as hemichannels, they promote ATP release, sustaining long-range ICS propagation; as GJ channels, they allow diffusion of Ca2+-mobilizing second messengers across coupled cells.
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