期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 40, 页码 15441-15445出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0807660105
关键词
biochemical mapping; immunofluorescence microscopy; in vitro aster formation; inhibitors of phosphorylation; phosphorylation
资金
- Howard Hughes Medical Institute
- Leukemia and Lymphoma Society
- Special Coordination Funds for Promoting Science and Technology (SCF)
- MEXT, Japan
Accurate mitotic chromosome segregation depends on the formation of a microtubule-based bipolar spindle apparatus. We report that the cohesin subunit structural maintenance of chromosomes subunit 1 (SMC1) is recruited to microtubule-bound RNA export factor 1 (Rae1) at the mitotic spindle pole. We locate the Rae1-binding site to a 21-residue-long region, SMC1(947-967) and provide several lines of evidence that phosphorylation of Ser(957) and Ser(966) of SMC1 stimulates binding to Rae1. Imbalances in these assembly pathways caused formation of multipolar spindles. Our data suggest that cohesin's known bundling function for chromatids in mitotic and interphase cells extends to microtubules at the spindle pole.
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