期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 36, 页码 13362-13366出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0802252105
关键词
fluorescence; high-pressure x-ray crystallography; protein engineering; protein structure-function; yellow fluorescent protein
资金
- Department of Energy Office of Biological and Envrionmental Research [FG02-97ER62443]
- National Institutes of Health Protein Structure [GM074899]
- National Institutes of Health
- National Science Foundation [DMR-0225180]
- National Institutes of Health [RR001646]
A protein molecule is an intricate system whose function is highly sensitive to small external perturbations. However, no examples that correlate protein function with progressive subangstrom structural perturbations have thus far been presented. To elucidate this relationship, we have investigated a fluorescent protein, citrine, as a model system under high-pressure perturbation. The protein has been compressed to produce deformations of its chromophore by applying a high-pressure cryocooling technique. A closely spaced series of x-ray crystallographic structures reveals that the chromophore undergoes a progressive deformation of up to 0.8 angstrom at an applied pressure of 500 MPa. It is experimentally demonstrated that the structural motion is directly correlated with the progressive fluorescence shift of citrine from yellow to green under these conditions. This protein is therefore highly sensitive to subangstrom deformations and its function must be understood at the subangstrom level. These results have significant implications for protein function prediction and biomolecule design and engineering, because they suggest methods to tune protein function by modification of the protein scaffold.
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