期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 28, 页码 9604-9609出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0802484105
关键词
force spectroscopy; generalized Rouse model; role of handles; reaction coordinate
资金
- National Research Foundation of Korea [R01-2008-000-10920-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The sequence-dependent folding landscapes of nucleic acid hairpins reflect much of the complexity of biomolecular folding. Folding trajectories, generated by using single-molecule force-clamp experiments by attaching semiflexible polymers to the ends of hairpins, have been used to infer their folding landscapes. Using simulations and theory, we study the effect of the dynamics of the attached handles on the handle-free RNA free-energy profile F-eq degrees(z(m)), where z(m) is the molecular extension of the hairpin. Accurate measurements of F-eq degrees(z(m)) requires stiff polymers with small L/I-p, where L is the contour length of the handle, and I-p is the persistence length. Paradoxically, reliable estimates of the hopping rates can only be made by using flexible handles. Nevertheless, we show that the equilibrium free-energy profile F-eq degrees(z(m)) at an external tension f(m), the force (f) at which the folded and unfolded states are equally populated, in conjunction with Kranners' theory, can provide accurate estimates of the force-dependent hopping rates in the absence of handles at arbitrary values of f. Our theoretical framework shows that z(m) is a good reaction coordinate for nucleic acid hairpins under tension.
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