期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 105, 期 19, 页码 6918-6923出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0801811105
关键词
chromosome motions; kinetochore-microtubule interactions; microtubule end tracking
资金
- NCRR NIH HHS [RR000592, P41 RR000592] Funding Source: Medline
- NIGMS NIH HHS [R01 GM033787, GM033787] Funding Source: Medline
Mitotic chromosomes segregate at the ends of shortening spindle microtubules (MTs). in budding yeast, the Dam1 multiprotein complex supports this dynamic attachment, thereby contributing to accurate chromosome segregation. Purified Dam1 will track the end of a depolymerizing MT and can couple it to microbead transport in vitro. The processivity of such motions has been thought to depend on rings that the Dam1 complex can form around MTs, but the possibility that alternative coupling geometries contribute to these motilities has not been considered. Here, we demonstrate that both rings and nonencircling Dam1 oligomers can track MT ends and enable processive cargo movement in vitro. The coupling properties of these two assemblies are, however, quite different, so each may make a distinct contribution to chromosome motility.
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