期刊
MBIO
卷 6, 期 1, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/mBio.02288-14
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资金
- U.S. Department of Energy (DOE), Office of Science, Office of Biological and Environmental Research's (OBER) Genomics Science Program to Lawrence Berkeley National Laboratory (LBNL)
- OBER Biological Systems Research on the Role of Microbial Communities in Carbon Cycling program [DE-SC0004601]
- U.S. National Science Foundation (NSF) MacroSystems Biology program [EF-1065844]
- NSF [CBET-1336709]
- Strategic Environmental Research and Development Program [ER-1587]
- National Institute of Environmental Health Sciences Superfund [P42ES004705]
- DOE Early Career Research Program [KP/CH57/1]
- DOE Office of Science [DE-AC02-05CH11231]
- Collaborative Innovation Center for Regional Environmental Quality
- State Key Joint Laboratory of Environment Simulation and Pollution Control at Tsinghua University
- NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [P42ES004705] Funding Source: NIH RePORTER
Understanding the structure, functions, activities and dynamics of microbial communities in natural environments is one of the grand challenges of 21st century science. To address this challenge, over the past decade, numerous technologies have been developed for interrogating microbial communities, of which some are amenable to exploratory work (e. g., high-throughput sequencing and phenotypic screening) and others depend on reference genes or genomes (e. g., phylogenetic and functional gene arrays). Here, we provide a critical review and synthesis of the most commonly applied open-format and closed-format detection technologies. We discuss their characteristics, advantages, and disadvantages within the context of environmental applications and focus on analysis of complex microbial systems, such as those in soils, in which diversity is high and reference genomes are few. In addition, we discuss crucial issues and considerations associated with applying complementary high-throughput molecular technologies to address important ecological questions.
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