期刊
PREVENTIVE VETERINARY MEDICINE
卷 116, 期 3, 页码 305-312出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.prevetmed.2014.01.021
关键词
Pools; Pancreas Disease; Salmonid alphavirus; Quantitative real-time polymerase chain-reaction; Virus isolation
资金
- Scottish Government
An experimental design and statistical analysis providing information on the reliability of pooled test procedures is described. It involves estimating the relationship between the probability of a positive pooled test result (dependent variable) and the expected number of infected individuals in a pool (explanatory variable). The intercept is an estimate of the proportion of false positives (1 - pooled specificity) and pooled sensitivities can be estimated for indicative prevalences of infected individuals. Simulations for a theoretical infection are used to investigate the advantages and limitations of the approach. The approach is used to evaluate the reliability of a virus isolation and qRT-PCR test procedure detecting Salmonid alphavirus the pathogenic agent necessary for the development of Pancreas Disease in Atlantic salmon (Salmo solar). Crown Copyright (C) 2014 Published by Elsevier B.V. All rights reserved.
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