期刊
PREVENTIVE VETERINARY MEDICINE
卷 89, 期 3-4, 页码 223-226出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.prevetmed.2009.02.020
关键词
Johne's disease; Food safety; Zoonosis
资金
- Cyprus Research Promotion Foundation [AEIFO 1104/02]
- Ministry of Agriculture of the Czech Republic [MZE0002716201, QH81065]
- EU [FOOD-CT-2005-007081]
The possibility that Mycobacterium avium subsp. paratuberculosis (MAP) plays some role in the development of Crohn's disease in humans is attracting attention to milk and milk products originating from infected animals. In this study, we focused on the detection of MAP in 220 bulk tank milk (BTM) samples from all dairy cattle herds in Cyprus. In total, 63 (28.6%) BTM milk samples were found to be positive for MAP using quantitative real-time PCR assays for IS900 and F57. The presence of MAP in BTM was low, and was assessed to be several tens of MAP cells per one ml of BTM. Milk samples examined by cultivation were found to be negative for MAP in all 220 BTM. In two BTM samples cultivation and subsequent sequencing of 16S rRNA revealed two isolates of M. fortuitum. (C) 2009 Elsevier B.V. All rights reserved.
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