期刊
JOURNAL OF FUNCTIONAL FOODS
卷 17, 期 -, 页码 761-773出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2015.06.033
关键词
Cocoa; Epicatechin; Hepatic HepG2 cells; Hepatic lipid deposition; Lipid metabolism; Type 2 diabetic ZDF rats
资金
- Spanish Ministry of Science and Innovation (MICINN) [AGL2010-17579, BFU2011-25420]
- Centro de Investigacion Biomedica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM, ISCIII)
Diabetes alters lipid metabolism that could lead to hepatic steatosis. The present study aimed to investigate the effect of a cocoa-enriched diet in type 2 diabetic Zucker diabetic fatty (ZDF) rats and that of cocoa-flavanol epicatechin (EC) in high-glucose-exposed HepG2 cells on hepatic lipid metabolism. Male ZDF cocoa-fed-rats had decreased body weight gain and improved circulating and hepatic lipid levels. Similarly, EC alleviated the altered lipid values induced in high-glucose-challenged HepG2 cells. The lipid-lowering effect was related to diminished fatty acid synthesis (sterol-regulatory-element-binding protein-1-c and fatty-acid synthase down-regulation), and increased fatty-acid oxidation (proliferator-activated-receptor a up-regulation). These effects depended on 5'-AMP-activated-protein-kinase (AMPK), protein kinase B (AKT) and protein kinase C (PKC)-zeta, which phosphorylated levels returned to control values upon cocoa or EC administration. Moreover, PKC zeta played a role on AKT and AMPK regulation. These findings suggest that cocoa and EC protect hepatocytes in vivo and in vitro by improving lipid metabolism through multiple-signalling pathways. (C) 2015 Elsevier Ltd. All rights reserved.
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