期刊
JOURNAL OF FUNCTIONAL FOODS
卷 19, 期 -, 页码 426-438出版社
ELSEVIER
DOI: 10.1016/j.jff.2015.08.030
关键词
Cytokines; GSH/GSSG; ROS; GSK3 beta; Homeostatic proliferation
资金
- Department of Atomic Energy, Government of India
A potent Nrf-2 activator sulforaphane (SF), which is currently under clinical trials for cancer therapy, was employed to elucidate the role of KeapVNrf-2/ARE signalling pathway in T-cell responses. SF suppressed mitogen induced T-cell and B-cell proliferation. It also inhibited mitogen induced upregulation of T-cell (CD25 and CD69) and B-cell (CD80 and CD86) activation markers and suppressed secretion of cytokines (IL-2, IL-4, IL-6 and IFN-gamma). SF induced oxidative stress and its anti-inflammatory effects were abrogated by thiol antioxidants. SF activated PI3K/AKT, leading to phosphorylation mediated suppression of GSK3 beta resulting in Nrf-2 activation. We also show for the first time that SF can prevent direct interaction between NF-kappa B and its consensus sequence by modulating thiol groups. It also suppressed homeostatic proliferation of T-cells and suppressed lipopolysaccharide induced proinflammatory mediators in macrophages. Our study shows that the potent anti-inflammatory effects of SF are mediated via modulation of PI3K/AKT/GSK3 beta/Nrf-2 and NF-kappa B pathway in T-cells. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据