期刊
POULTRY SCIENCE
卷 91, 期 6, 页码 1326-1334出版社
OXFORD UNIV PRESS
DOI: 10.3382/ps.2011-02026
关键词
interleukin-4; in ovo vaccination; coccidiosis
Two experiments were conducted to study the effects of an in ovo interleukin (IL)-4 plasmid injection in a coccidia infection model. In experiment I; chicks were hatched from eggs that had been injected in ovo with an empty vector or with 10 or 15 mu g of IL-4 plasmid, and then challenged posthatch with coccidia. In experiment II, chicks were hatched from eggs that had been vaccinated with coccidia and injected in ovo with an empty vector or with 10 or 15 mu g of IL-4 plasmid, and then challenged posthatch with coccidia. In experiment II, the BW gain of birds hatched from eggs vaccinated with live oocysts plus 15 mu g of IL-4 plasmid was 25% higher than the BW gain of birds hatched from eggs vaccinated with live oocysts plus empty plasmid. In both experiments I and II, a 15-mu g IL-4-plasmid injection decreased fecal oocyst shedding, decreased the number of CD8(+) cells in the cecal tonsils; and decreased cecal tonsil lymphocyte cell proliferation postcoccidia challenge. In experiment I, splenic macrophages of chicks hatched from eggs injected with 15 mu g of IL-4 plasmid had higher nitric oxide production than those of chicks hatched from eggs injected with the empty plasmid. In experiment II, a 15-mu g IL-4-plasmid injection increased serum anticoccidia IgG postcoccidia challenge. It could be concluded that 15 mu g of IL-4 plasmid improved anticoccidia immune responses synergistically with in ovo coccidia vaccination in chickens
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据