Effects of divergent selection for 8-week body weight on postnatal enzyme activity pattern of 3 fiber types in fast muscles of male broilers (Gallus gallus domesticus)

A divergent selection experiment was conducted for 8-wk BW in chickens. At 3, 6, 9, and 12 wk of age, samples of pectoralis profundus (PP) and biceps femoris (BF) muscles from fast-growing and slow-growing lines were used to estimate the enzyme activities and muscle fiber diameter. Microphotometric measurements made in situ of succinate dehydrogenase (SDH, EC 1.3.99.1) and glycerol-3-phosphate dehydrogenase (GPDH, EC 1.1.99.5) were completed on serial sections of PP and BF muscles from male chickens, in order to examine the ratio of SDH: GPDH activity in single fibers. On the basis of the SDH: GPDH activity ratios, muscle fibers were divided using cluster analysis into 3 populations of different fiber types (O = oxidative, OG = oxidative-glycolytic, and G = glycolytic). Cockerels of the SGL attained an 8.1-fold increase and those of the FGL a 6.8-fold increase in BW at 12 wk compared with that at 3 wk of age. The O, OG, and G type fibers of the BF muscles of the SGL had significantly (P = 0.001) lower SDH: GPDH activity ratios than those of the FGL. A step decrease in the SDH: GPDH activity of O, OG, and G fibers in the PP of both lines occurred, and this differed significantly between SGL and FGL (P = 0.001). Age and line effects influenced the diameter of the 3 fiber types in the BF muscle only. In contrast to this response, all 3 fiber types of the PP muscles reached similar diameters in both lines during the growth process from wk 3 to 12. From the results of this study, we concluded that the activities of metabolic enzymes in skeletal muscle fibers are under the influence of muscle type, age, and selection pressure. Micro-photometry is a suitable method for the evaluation of enzyme activity measured in a single muscle fiber. The method enables precise estimation of enzyme activities, especially in muscles composed of populations of different metabolic fiber types.