期刊
POSTHARVEST BIOLOGY AND TECHNOLOGY
卷 86, 期 -, 页码 212-220出版社
ELSEVIER
DOI: 10.1016/j.postharvbio.2013.07.003
关键词
ACC; ACO; ACS; Chilling; Ethylene receptors; Gene expression
资金
- University of Lleida
To further understand the response of 'Conference' pears to 1-methylcyclopropene (1-MCP) treatment and their ability to restore ripening after prolonged periods of cold storage, fruit were treated with 0, 300 nL L-1 1-MCP or 300 nL L-1 exogenous ethylene plus 600 nL L-1 1-MCP prior to storage. Changes in ethylene, ethylene precursors (ACC, MACC), ethylene-related enzyme activities (ACS, ACO) together with their transcript levels, and the expressioii of four ethylene receptors and one Raf kinase protein from the ethylene signalling pathway, were monitored before and after cold storage and during subsequent ripening at 20 degrees C. 1-MCP treatment acted on the ethylene pathway in two differentiated phases. In a first initiation occurring during cold storage, the 1-MCP treatment limited the up-regulation of both PcACS1 and PcACO1 observed in control fruit and promoted an up-regulation of PcETR1 leading to a complete inhibition of ACO activity during cold storage. These regulations resulted in fruit unable to produce ethylene upon removal and promoted the second phase (maintenance phase). This second phase was characterized by a dawn-regulation of PcACS1 and PcACS4 as well as PcACO1 together with a clear up-regulation of PcETR5 and better maintenance of PcCTR1 transcript levels, which were partially reversed with exogenous ethylene treatment. All these different regulations led in turn to a complete inhibition of the ripening processes, which may partially explain the occurrence of the evergreen behaviour in 'Conference' pear during shelf-life. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据