期刊
POSTHARVEST BIOLOGY AND TECHNOLOGY
卷 78, 期 -, 页码 113-122出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.postharvbio.2012.12.013
关键词
Green mould; Blue mould; Maturity stage; Wound response; Lignin; Phenylpropanoid pathway
资金
- Spanish Government [AGL2008-04828-C03/AGR, AGL2011-30519-C03/AGR]
- 'Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria' (INIA)
Penicillium digitatum is the most devastating postharvest pathogen of citrus. In addition, Penicillium expansum is the main pathogen of pome fruit, although recent studies have demonstrated its ability to infect oranges under some conditions. In this study, we evaluated wound response in 'Valencia' oranges harvested at three different maturity stages and the effect of wound response on the establishment of both pathogens when fruit were stored at two different temperatures (20 and 4 degrees C). The effect of wounding and pathogen inoculation on lignin content, was also quantified. Lastly, the expression of several phenylpropanoid pathway-related genes was also analyzed by semi-quantitative RT-PCR. Results indicated that, in general, P. digitatum exhibited lower decay incidence and severity as time between wounding and inoculation increased. Decay incidence and severity were higher in fruit from the over-mature harvest than in fruit from immature and commercial harvests. P. expansum was able to infect fruit at 20 degrees C but lesions were small compared to lesion size of fruit stored at 4 degrees C. Lignin content in wounded fruit (control) and in samples wounded and inoculated with P. expansum was highest in fruit from the immature harvest at 7 d post-wounding and inoculation. Wounded fruit had higher expression of pall, comtl and pox1 genes at 48 h than at 24 h. However, samples inoculated with P. digitatum showed lower expression at 48 h than at 24 h. Our results indicated that maturity and storage temperature play an important role in orange wound response. (C) 2013 Elsevier B.V. All rights reserved.
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