期刊
POSTHARVEST BIOLOGY AND TECHNOLOGY
卷 58, 期 1, 页码 48-56出版社
ELSEVIER
DOI: 10.1016/j.postharvbio.2010.05.006
关键词
Gladiolus; Expansin; Cell expansion; Gibberellin; Promoter; GUS
资金
- Indian National Science Academy
- Department of Biotechnology, India
- Council for Scientific and Industrial Research, New Delhi, India
The final shape and size of the flower is genetically and developmentally controlled by tight regulation of cell number and cell size with cell expansion playing an important role The gladiolus expansin gene, GgEXPA1, was expressed prominently during phases of active tepal expansion and cell elongation in stamen filaments, gynoecium styles and expanding leaves but not in tissues where expansion had ceased and senescence had been initiated. Within tepals, differential expression between the proximal and distal portions that differ in cell elongation was observed. The expression of the gene was responsive to GA and inhibited by the GA biosynthesis inhibitor, paclobutrazol. The promoter of GgEXPA1 showed strong expansion-responsive GUS expression in young agro-infiltrated gladiolus tepals and in etiolated hypocotyls and light grown expanding cotyledonary leaves of transgenic Arabidopsis seedlings. Inhibition of hypocotyl elongation by paclobutrazol blocked the expression of the promoter-driven reporter gene indicating GA responsiveness of the promoter. GgEXPA1 provides an interesting example of a single expansin gene being involved in expansion processes in different plant tissues such as tepals, stamens, pistils and leaves that are both spatially as well as temporally distinct in their development. The studies provide a basis for GA mediated expansion of floral organs via expansins prior to anthesis. (C) 2010 Elsevier B.V. All rights reserved.
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