期刊
POSTHARVEST BIOLOGY AND TECHNOLOGY
卷 52, 期 1, 页码 16-23出版社
ELSEVIER
DOI: 10.1016/j.postharvbio.2008.09.010
关键词
Zucchini squash; Cold storage; Membrane fluidity; Reactive oxygen species; Scavenging enzymes; Alternative oxidase
资金
- MIUR Italy and of Polytechnic University of Marche
- Dipartimento di Protezione e Valorizzazione Agro-alimentare di Bologna
Steady-state levels of hydrogen peroxide and activities of catalase and peroxidase were measured in the peel and pulp of zucchini squash (Cucurbita pepo L., cv., Giambo) during storage at 10 degrees C or 0 degrees C for two weeks. No visible damage occurred during this storage time; epidermal brown pits become visible later, at day 20 in fruit stored at 10 degrees C and at day 15 in those stored at 0 degrees C. In order to analyze the early effects of the chilling-induced oxidative stress during zucchini storage, rates of succinate oxidation, alternative oxidase activity, membrane fluidity and phospholipid composition were also measured in mitochondria isolated from the zucchini pulp. A decrease in hydrogen peroxide levels, an increase in the activity of detoxifying enzymes, a recovery of chilling-induced mitochondrial membrane fluidity and an increase in alternative oxidase (AOX) activity were detected in the early stages of zucchini storage at 10 degrees C. The peroxidase and the AOX activities of the pulp of zucchini stored at 0 degrees C were also found to increase but to a lesser extent and later during storage, suggesting that these fruit can also activate these ROS regulatory systems, possibly preventing the occurrence of early visible damage in the peel but not the occurrence of cold stress. (C) 2008 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据