期刊
POLYMERS FOR ADVANCED TECHNOLOGIES
卷 22, 期 12, 页码 1925-1930出版社
WILEY-BLACKWELL
DOI: 10.1002/pat.1694
关键词
gene therapy; poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA); polyesters; self-assembly; thermo-sensitive polymers
资金
- 973 Program [2009CB930300]
- Tianjin Municipal Natural Science Foundation [08JCZDJC17200, 08JCYBJC01800, 09JCYBJC13800]
- National Natural Science Foundation of China [30672554]
- Specialized Research Fund for the Doctoral Program of Higher Education [20090032120013]
- Tianjin University Youth Teacher Culture Foundation [TJU-YFF-08B15]
Polycaprolactone-graft-Poly(2-(dimethylamino)ethyl methacrylate-co-methoxy polyethylene glycol monomethacrylate) (PCL-graft-P(DMAEMA-co-mPEGMMA)) was synthesized by combination of ring-opening polymerization (ROP) and atom transfer radical polymerization (ATRP). PCL-graft-P(DMAEMA-co-mPEGMMA) was characterized by FTIR, (1)H NMR, and GPC. PCL-graft-P(DMAEMA-co-mPEGMMA) with expected composition and structure was achieved. pH-and thermo-sensitive properties of the PCL-graft-P(DMAEMA-co-mPEGMMA) nanoparticles prepared by the nanoprecipitation method were investigated by TEM and DLS. With increase in the temperature, the size of PCL-graft-P(DMAEMA-co-mPEGMMA) nanoparticles is decreased under base environment. Furthermore, in vitro transfection and toxicity assays were tested in 293T cells. The results indicate that PCL-graft-P(DMAEMA-co-PEGMMA) has lower cytotoxicity at N/P ratios less than 10 with transfection efficiency concomitantly reducing at N/P ratios less than 20 compared to PCL-graft-PDMAEMA as the control. However, PCL-graft-P(DMAEMA-co-PEGMMA) presents higher transfection efficiency at N/P ratios more than 20 compared to PCL-graft-PDMAEMA. Copyright (C) 2010 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据