期刊
MEDCHEMCOMM
卷 6, 期 3, 页码 455-460出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4md00495g
关键词
-
资金
- Japan Society for the Promotion of Science (JSPS)
- Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)
- Naito Foundation
- Tokyo Biochemical Research Foundation
- Workshop on Chirality in Chiba University (WCCU)
- Canadian Institutes of Health Research (CIHR) with the Canadian Blood Services (CBS)
- CIHR
- Grants-in-Aid for Scientific Research [15H04650, 23102008] Funding Source: KAKEN
The isolation and evaluation of inhibitors of Fanconi F protein (FANCF) - haity and enhancer of split 1 (Hes1) complex are described. A high-throughput screening assay for small-molecule inhibitors of the FANCF-Hes1 complex was realized. Successful complex formation between fluorophore (Cy3)-labeled human FANCF and immobilized rat or human HES1 on a microplate was established. Screening of our plant extract library using this system resulted in the isolation of eight natural products, including two new flavonoid glycosides (3 and 4), from Wrightia religiosa. Of these compounds, 3, 5, and 7 showed potent inhibition of the FANCF-Hes1 complex formation. Compound 7 disrupted the FANCF-Hes1 complex more efficiently than the Hes1 dimer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据