Electrospinning of Asiaticoside/2-Hydroxypropyl-β-cyclodextrin Inclusion Complex-loaded Cellulose Acetate Fiber Mats: Release Characteristics and Potential for Use as Wound Dressing

Cellulose acetate (CA) fiber mats containing inclusion complexes of asiaticoside (AC) in 2-hydroxypropyl-beta-cyclodextrin (HP beta CD) for potential usage as wound dressings were developed. The AC/HP beta CD complex-loaded CA fibers at various HP beta CD to AC molar ratios of 0.5, 1, and 2 were prepared in 90:10 v/v mixture of 80% (v/v) acetic acid and N,N-dimethylacetamide (DMAc) via electrospinning. The maximum released amounts of AC depended on the HP beta CD content and were much greater than those released from the AC-loaded CA fiber mat In the in vitro study, indirect cytotoxic evaluation with human dermal fibroblasts (HDFa) showed that these materials released no substances in the levels that were harmful to the cells and the cells appeared to attach and proliferate well on these substrates. However, only the CA fiber mats containing AC/HP beta CD complexes at the HP beta CD to AC molar ratio of 0.5 was effective in up-regulating the production of collagen of the cultured cells.