Development of silver nanoparticles-loaded calcium alginate beads embedded in gelatin scaffolds for use as wound dressings

Silver nanoparticles (AgNPs)-loaded calcium alginate beads embedded in gelatin scaffolds were developed to sustain and maintain the release of silver (Ag+) ions over an extended time period. The UV irradiation technique was used to reduce Ag+ ions in alginate solution to AgNPs. The average sizes of AgNPs ranged between ca 20 and ca 22nm. The AgNPs-loaded calcium alginate beads were prepared by electrospraying of a sodium alginate solution containing AgNPs into calcium chloride (CaCl2) solution. The AgNPs-loaded calcium alginate beads were then embedded into gelatin scaffolds. The release characteristics of Ag+ ions from both the AgNPs-loaded calcium alginate beads and the AgNPs-loaded calcium alginate beads embedded in gelatin scaffolds were determined in either deionized water or phosphate buffer solution at 37 degrees C for 7 days. Moreover, the AgNPs-loaded calcium alginate beads embedded in gelatin scaffolds were tested for their antibacterial activity and cytotoxicity. (c) 2014 Society of Chemical Industry