期刊
POLYMER DEGRADATION AND STABILITY
卷 109, 期 -, 页码 293-306出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.polymdegradstab.2014.07.017
关键词
Polycaprolactone; Metronidazole; Gelatin; Electrospinning; Guided tissue regeneration
资金
- National Natural Science Foundation of China [51303014, 81330043]
- Beijing Nova Program [Z131102000413015]
- National Outstanding Youth Science Fund [50725310]
- Ministry of Science and Technology of the China [2012BAI10B02]
- Beijing Municipal Training Programme Foundation for the Talents [2013D00303400041]
Nanofiber membranes composed of polycaprolactone (PCL), PCL/metronidazole (MNA), PCL/gelatin/MNA, and PCL/gelatin/MNA/acetic acid (HAC), named PO, P30, PG30, and PGH30, respectively, were fabricated by electrospinning for application in guided tissue/bone regeneration (GTR/GBR) therapies. The architectural features, mechanical properties, hydrophilicity, drug-encapsulation efficiency, drug-release pattern, antimicrobial properties, cell barrier functions, in vitro/vivo degradability and biocompatibility were investigated. All membranes were found to have high tensile strength, which is required for GTR applications. Strong interactions among PCL, gelatin, and MNA resulted in high drug loading efficiency, which was further improved by the incorporation of gelatin and HAC. MNA incorporation gave the membranes good antimicrobial property, while reducing host versus graft reaction, improving the hydrophilicity and accelerating the degradation. Gelatin incorporation considerably improved cytocompatibility, while accelerating the degradation dramatically. Very low quantities (0.1% v/v with respect to polymer solution) of HAC effectively prevented the phase separation of PCL and gelatin, resulting in homogeneous nanofiber, which facilitates stable physical properties. The drug-release profiles of all drug-loading membranes were consistent with the inflammation cycle characteristics. High drug loading and trace amounts of HAC did not cause any adverse reactions, as evidenced by subcutaneous implantation. Both PO and P30 maintained their cell barrier function in vivo for as long as 24 weeks; PGH30, for 8 weeks; and PG30, for less than 8 weeks. These findings enabled a comprehensive understanding of the influence of different compositions on the structure and performance of the membranes, thereby supporting the design of membranes with superior overall performance for GTRIGBR application. (C) 2014 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据