期刊
POLYMER DEGRADATION AND STABILITY
卷 95, 期 8, 页码 1429-1435出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.polymdegradstab.2010.01.002
关键词
Polyhydroxybutyrate; PHA; PHB depolymerase; PHB binding domain
The 3-dimensional structure of the Paucimonas lemoignei poly(3-hydroxybutyrate) (PHB) depolymerase PhaZ7 has significant similarity to Bacillus subtilis lipase LipA but differs from the latter by the presence of an additional domain. Analysis of this lid-like domain revealed the presence of many hydrophobic amino acid residues including Tyr(105). In this study we constructed His-tag fusions of PhaZ7 for simplified purification and investigated the effect of amino acid exchange of eight tyrosine codons of the lid-like domain. Exchanges of Tyr(103), Tyr(172), Tyr(173), Tyr(203) or Tyr(204) to alanine or serine had no phenotype but muteins with substitution of Tyr(189), Tyr(190) and Tyr(105) to alanine showed a lag phase of the in vitro PHB depolymerase reaction. Replacement of Tyr(105) by glutamate further increased the lag phase. Binding assays of the purified PHB depolymerase proteins with the natural substrate, native PHB granules, revealed a significantly reduced binding ability of the Tyr(105)Glu mutant compared to the wild type protein and confirmed that Tyr(105) is involved in interaction with the polymeric substrate. (C) 2010 Elsevier Ltd. All rights reserved.
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