期刊
POLYMER
卷 53, 期 8, 页码 1684-1693出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.polymer.2012.02.031
关键词
Star block copolymer; Thermoresponsive micelle; Specific target recognition
资金
- Fundamental Research Funds for the Central Universities [WD0913008, WD1014017]
- Shanghai Municipal Education Commission
- Natural Science Foundation of Shanghai [11ZR1409200]
- National Natural Science Foundation of China [51103041]
- Shanghai Leading Academic Discipline Project [B502]
- Shanghai Key Laboratory [08DZ2230500]
- Changjiang Scholars and Innovative Research Team in University [IRT0825]
A novel star amphiphilic block copolymer star poly(epsilon-caprolactone)-b-poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide)-DDAT [SPCL-b-P(NIPAAm-co-DMAAm)-DDAT] (DDAT: S-1-dodecyl-S'-(alpha,alpha'-dimethyl-alpha-acetic acid)trithiocarbonate) was synthesized by combination of ring-opening polymerization (ROP) and reversible addition-fragment chain transfer (RAFT) polymerization. DDAT-terminated groups were further transformed into hydroxyl groups by one-pot strategy for aminolysis of DDAT and Michael addition reaction of an alpha,beta-unsaturated ester of 2-hydroxyethyl acrylate (HEA). Biotinylated star copolymer SPCL-b-P(NIPAAm-co-DMAAm)-Biotin was obtained by coupling of biotin to the hydroxyl-terminated star copolymer using carbodiimide coupling chemistry. These star copolymers with DDAT, hydroxyl, and biotin end groups were capable of self assembling into core-shell structural micelles in aqueous solution. The variation of end groups significantly affected the micellar characters, such as hydrodynamic diameter (D-h), critical micellar concentration (CMC), and lower critical solution temperature (LCST). Biotinylated micelle exhibited a phase transition at 41.4 degrees C. The amount of biotin on the micelle surface as well as the specific recognition between biotinylated micelle and avidin was determined by 4'-hydroxyazobenzene-2-carboxylic acid/avidin (HABA/avidin) binding assay and dynamic light scattering (DLS). In addition, the biotinylated star copolymer displayed good biocompatibility according to a preliminary cytotoxicity study. The novel polymeric micelle with biodegradability, thermoresponse, and specific target recognition was expected to be a promising polymeric carrier material for targeted drug delivery. (C) 2012 Elsevier Ltd. All rights reserved.
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