期刊
POLYMER
卷 49, 期 19, 页码 4180-4187出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.polymer.2008.07.044
关键词
FRET; pH; polysulfonamide
资金
- Ministry of Science and Engineering Foundation (MOST)
- National Research Foundation of Korea [과C6A2003, 2007-00015] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A fluorescence resonance energy transfer (FRET) sensor, which has a FRET donor and an acceptor attached to each chain end of pH-sensitive polysulfonamide, is synthesized and its pH sensitivity is examined in terms of the FRET efficiency. This polymeric sensor exhibits an instantaneous conformation change from coil to globule at a specific pH, which results in the drastic on-and-off FRET efficiency. To detect a specific pH region, sulfadimethoxine and sulfamethizole are selected among various sulfonamides since their pK(a) values are in the physiological pH. For tuning the emission color arising from FRET, 7-hydroxy-4-bromomethyl coumarin and coumarin 343 are used as a FRET donor and an acceptor, respectively, for a blue-to-green FRET sensor, and fluorescence amine isomer I and rhodamine B are used for a green-to-red FRET sensor. Each sensor shows a distinct color change from the emission wavelength of FRET donor to the emission wavelength of FRET acceptor, which well explains their feasibility as a useful optical sensor. (C) 2008 Elsevier Ltd. All rights reserved.
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