期刊
POLISH JOURNAL OF CHEMICAL TECHNOLOGY
卷 15, 期 2, 页码 94-98出版社
DE GRUYTER POLAND SP ZOO
DOI: 10.2478/pjct-2013-0029
关键词
Ionic liquid; [BMIM]Cl; toxicity; Saccharomyces cerevisiae AY93161; lignocellulosic ethanol production
资金
- National Natural Science Foundation of China [21176196]
- Graduate Innovative Fund of Wuhan Institute of Technology [CX201203]
- Key Laboratory for Green Chemical Process of Ministry of Education [GCP201206]
Ionic liquid (IL) pretreatment of lignocellulosic materials has provided a new technical tool to improve lignocellulosic ethanol production. To evaluate the influence of the residual IL in the fermentable sugars from enzymatic hydrolysis of IL pretreatment of lignocellulosic materials on the subsequent ethanol fermentation, the toxicity of the IL 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) to Saccharomyces cerevisiae AY93161 was investigated. Firstly, the morphological structure, budding and metabolic activity of Saccharomyces cerevisiae AY93161 at different [BMIM]Cl concentrations were observed under an optical microscope. The results show that its single cell morphology remained unchanged at all [BMIM]Cl concentrations, but its reproduction rate by budding and its metabolic activity decreased with the [BMIM]Cl concentration increasing. The half effective concentration (EC50) and the half inhibition concentration (IC50) of [BMIM]Cl to Saccharomyces cerevisiae AY93161 were then measured using solid and liquid suspension culture and their value were 0.53 and 0.39 gL(-1) respectively. Finally, the influence of [BMIM]Cl on ethanol production was investigated. The results indicate that the [BMIM] Cl inhibited the growth and ethanol production of Saccharomyces cerevisiae AY93161. This toxicity study provides useful basic data for further development in lignocellulosic ethanol production by using IL technology and it also enriches the IL toxicity data.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据