期刊
POLAR BIOLOGY
卷 36, 期 11, 页码 1577-1586出版社
SPRINGER
DOI: 10.1007/s00300-013-1375-8
关键词
Polar; Arctic; Ecosystem; NMR; LC-MS
资金
- SINTEF internal fund (Konsernsatsning Calanomics)
- Research Council of Norway [196711/S40]
- Svalbard Science Forum
Recent development in high-throughput unbiased analytical tools (transcriptomics, proteomics and metabolomics) has opened the possibility to assess a multitude of molecular endpoints in organisms. In the present work, we used a combination of metabolomics tools, proton nuclear magnetic resonance spectroscopy (H-1-NMR) and liquid chromatography coupled with mass spectrometry (LC-MS),) and multivariate statistical analysis to identify species-specific metabolome fingerprints and species-specific metabolites in the three Arctic copepods Calanus finmarchicus, C. glacialis and C. hyperboreus. Principal component analysis separated the three species with high specificity and sensitivity, and some species-specific metabolites were putatively annotated. These tools can be used for future studies within basal biology, systems biology, bioprospecting and ecotoxicology. As a supplementary analytical tool to genetic analyses, species-specific metabolites have a potential to be used to separate closely related Arctic Calanus species from net hauls.
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