期刊
PLOS ONE
卷 12, 期 4, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0175146
关键词
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资金
- Belgian Politique Scientifique Federale program [IAP P7/44 iPROS]
- FRFC program of the Belgian National Fund for Scientific Research F.R.S- FNRS [2.4522.12]
- Universite A catholique de Louvain
Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and short overlapping ends. Our method called QuickLib should find many applications in synthetic biology; as an example, we easily prepared genetic libraries of Escherichia coli expressing billions of different backbone cyclic peptides.
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