4.6 Article

Comparison of Zebrafish tmem88a mutant and morpholino knockdown phenotypes

期刊

PLOS ONE
卷 12, 期 2, 页码 -

出版社

PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0172227

关键词

-

资金

  1. Cancer Research UK [FC001-157]
  2. UK Medical Research Council [FC001-157]
  3. Wellcome Trust [FC001-157]
  4. Fondation Leducq
  5. Francis Crick Institute
  6. MRC [MC_U117597140] Funding Source: UKRI
  7. Medical Research Council [MC_U117597140] Funding Source: researchfish
  8. The Francis Crick Institute [10158] Funding Source: researchfish

向作者/读者索取更多资源

Tmem88a is a transmembrane protein that is thought to be a negative regulator of the Wnt signalling pathway. Several groups have used antisense morpholino oligonucleotides in an effort to characterise the role of tmem88a in zebrafish cardiovascular development, but they have not obtained consistent results. Here, we generate an 8 bp deletion in the coding region of the tmem88a locus using TALENs, and we have gone on to establish a viable homozygous tmem88a Delta 8 mutant line. Although tmem88a Delta 8 mutants have reduced expression of some key haematopoietic genes, differentiation of erythrocytes and neutrophils is unaffected, contradicting our previous study using antisense morpholino oligonucleotides. We find that expression of the tmem88a paralogue tmem88b is not significantly changed in tmem88a8,8 mutants and injection of the tmem88a splice-blocking morpholino oligonucleotide into tmem88a Delta 8 mutants recapitulates the reduction of erythrocytes observed in morphants using o-Dianisidine. This suggests that there is a partial, but inessential, requirement for tmem88a during haematopoiesis and that morpholino injection exacerbates this phenotype in tmem88a morpholino knockdown embryos.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据