期刊
CHEMICAL SCIENCE
卷 6, 期 1, 页码 745-751出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4sc01953a
关键词
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资金
- Ministry of Science and Technology [2011CB809100]
- NSFC [21272016, 21002003, 91013003, J1030413]
- Ministry of Education of China [20120001110083]
Characterization of transcription factor-DNA interaction is of high importance in elucidating the molecular mechanisms of gene transcriptions. DNA-based affinity probes were developed to capture and identify transcription factors by covalent crosslinking; however, the requirement of a crosslinker on the affinity probe remains a disadvantage, as the crosslinker itself often interferes with the protein-DNA interactions. We report a dual-probe method able to capture DNA-binding transcription factors with unmodified protein-binding sites in scenarios where conventional probes have failed. We have also shown the method's converse application in selecting specific transcription factor-binding DNA sequences from a probe library and its extension to studying proteins recognizing epigenetic marks. This study may provide a new tool for exploring DNA-binding proteins in biology.
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