期刊
PLOS ONE
卷 11, 期 7, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0159399
关键词
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资金
- Ministry of Spain - MINECO (Fondos Europeos de Desarrollo Regional de la Union Europea [FEDER]) [SAF2012-32491, SAF2013-40987R]
- Centro de Investigacion Biomedica en Red Fisiopatologia de la Obesidad y la Nutricion (CIBEROBN) [CB06/03/0001]
- Centro de Investigacion Biomedica en red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM)
- Generalitat de Catalunya [2014SGR465, 2014SGR48]
- Comunidad de Madrid [S2010/BMD-2423]
- INTERREG IV-B-SUDOE-FEDER (DIOMED) [SOE1/P1/E178]
- European Foundation for the Study of Diabetes (EFSD)/Janssen-Rising Star research fellowship
- ICREA-Academia (Generalitat de Catalunya)
- Ciencia sem Fronteiras-CNPq fellowship [237976/2012-9]
- European Foundation for the Study of Diabetes (EFSD)/L'Oreal-UNESCO For Women in Science research fellowship
- Ministry of Spain - MINECO [SAF2013-45887-R, SAF2014-52223-C2-1-R, SAF-2015-65267-R]
The discovery of active brown adipose tissue (BAT) in adult humans and the fact that it is reduced in obese and diabetic patients have put a spotlight on this tissue as a key player in obesity-induced metabolic disorders. BAT regulates energy expenditure through thermogenesis; therefore, harnessing its thermogenic fat-burning power is an attractive therapeutic approach. We aimed to enhance BAT thermogenesis by increasing its fatty acid oxidation (FAO) rate. Thus, we expressed carnitine palmitoyltransferase 1AM (CPT1AM), a permanently active mutant form of CPT1A (the rate-limiting enzyme in FAO), in a rat brown adipocyte (rBA) cell line through adenoviral infection. We found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced disorders.
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