期刊
PLOS ONE
卷 11, 期 3, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0152683
关键词
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资金
- National Institutes of Health [5UL1DE019581, RL1DE019021, 1K01DK101684-01, 1U01HG007037, 5P01NS055923]
- Harvard Stem Cell Institute's Sternlicht Director's Fund award
- Human Frontier Science Program grant
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding.
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