期刊
PLOS ONE
卷 10, 期 9, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0137017
关键词
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资金
- Ministry of Science and Technology of Taiwan [MOST 103-2633-B-002-014, NSC 102-2320-B-002-002-MY2, NSC 101-2628-E-002-034-MY3, NSC-DAAD 102-2911-I-002-507]
- National Taiwan University Hospital-Taipei Veterans General Hospital [NTUH-TVGH VN103-10]
- National Taiwan University [102R7745, 103R7745]
The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications. In this study, a simple and effective method was designed to immobilize biomolecules via amine-N-hydroxysuccinimide (NHS) ester conjugation reaction using functionalized poly-p-xylylene coating on material surfaces. The NHS ester functionalized coating is synthesized via chemical vapor deposition, a facile and solvent-less method, creating a surface which is ready to perform a onestep conjugation reaction. Bone morphogenetic protein 2 (BMP-2) is immobilized onto material surfaces by this coating method, forming an osteogenic environment. The immobilization process is controlled at a low temperature which does not damage proteins. This modified surface induces differentiation of preosteoblast into osteoblast, manifested by alkaline phosphatase (ALP) activity assay, Alizarin Red S (ARS) staining and the expression of osteogenic gene markers, Alp! and Bglap3. With this coating technology, immobilization of growth factors onto material surface can be achieved more simply and more effectively.
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