期刊
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE
卷 8, 期 8, 页码 646-651出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.1016/j.apjtm.2015.07.008
关键词
Exercise preconditioning; Exhaustive exercise; Myocardial injury; Cardioprotection; Connexin 43
资金
- Chinese Postdoctoral Science Foundation [2014N560538]
- Hainan Province Colleges and Universities Scientific Research Project [Hnky2015-34]
- Natural Science Foundation of Hainan Province [314090]
Objective: To investigate the expression of myocardium connexin 43 (Cx43) in late exercise preconditioning (LEP) cardioprotection. Methods: Eight-week-old adult male Sprague Dawley rats were randomly assigned into four groups (n=8). Myocardial injury was judged in accordance with serum levels of cTn I and NT-proBNP as well as hematoxylin basicfuchsin picric acid staining of myocardium. Cx43 mRNA was detected by in situ hybridization and qualified by real-time fluorescence quantitative PCR. Cx43 protein was localized by immunohistochemistry and its expression level was determined by western blotting. Results: The LEP obviously attenuated the myocardial ischemia/hypoxia injury caused by exhaustive exercise. There was no significant difference of CA:113 mRNA level between the four groups. Cx43 protein level was decreased significantly in group EE (P<0.05). However, LEP produced a significant increase in Cx43 protein level (P<0.05), and the decreased Cx43 protein level in exhaustive exercise was significantly up-regulated by LEP (P<0.05). Conclusions: LEP protects rat heart against exhaustive exercise-induced myocardial injury by up-regulating the expression of myocardial Cx43.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据