期刊
PLOS ONE
卷 10, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0115515
关键词
-
资金
- National Institute of Diabetes and Digestive and Kidney Diseases [RO1 DK071014, RO1 DK73070]
- American Heart Association MWA Affiliate [11PRE7530018]
A low Na, high K diet (LNaHK) is associated with a low rate of cardiovascular (CV) disease in many societies. Part of the benefit of LNaHK relies on its diuretic effects; however, the role of aldosterone (aldo) in the diuresis is not understood. LNaHK mice exhibit an increase in renal K secretion that is dependent on the large, Ca-activated K channel, (BK-alpha with accessory BK-beta 4; BK-alpha/beta 4). We hypothesized that aldo causes an osmotic diuresis by increasing BK-alpha/beta-mediated K secretion in LNaHK mice. We found that the plasma aldo concentration (P[aldo]) was elevated by 10-fold in LNaHK mice compared with control diet (Con) mice. We subjected LNaHK mice to either sham surgery (sham), adrenalectomy (ADX) with low aldo replacement (ADX-LA), or ADX with high aldo replacement (ADX-HA). Compared to sham, the urinary flow, K excretion rate, transtubular K gradient (TTKG), and BK-alpha and BK-beta 4 expressions, were decreased in ADX-LA, but not different in ADX-HA. BK-beta 4 knockout (beta 4KO) and WT mice exhibited similar K clearance and TTKG in the ADX-LA groups; however, in sham and ADX-HA, the K clearance and TTKG of beta 4KO were less than WT. In response to amiloride treatment, the osmolar clearance was increased in WT Con, decreased in WT LNaHK, and unchanged in beta 4KO LNaHK. These data show that the high P[aldo] of LNaHK mice is necessary to generate a high rate of BK-alpha/beta 4-mediated K secretion, which creates an osmotic diuresis that may contribute to a reduction in CV disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据