4.6 Article

Higher Protein Kinase C ζ in Fatty Rat Liver and Its Effect on Insulin Actions in Primary Hepatocytes

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PLOS ONE
卷 10, 期 3, 页码 -

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PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0121890

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  1. Allen Foundation Inc
  2. University of Tennessee at Knoxville
  3. American Heart Association [09SDG2140003]
  4. China Scholarship Council

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We previously showed the impairment of insulin-regulated gene expression in the primary hepatocytes from Zucker fatty (ZF) rats, and its association with alterations of hepatic glucose and lipid metabolism. However, the molecular mechanism is unknown. A preliminary experiment shows that the expression level of protein kinase C zeta (PKC zeta), a member of atypical PKC family, is higher in the liver and hepatocytes of ZF rats than that of Zucker lean (ZL) rats. Herein, we intend to investigate the roles of atypical protein kinase C in the regulation of hepatic gene expression. The insulin-regulated hepatic gene expression was evaluated in ZL primary hepatocytes treated with atypical PKC recombinant adenoviruses. Recombinant adenovirus-mediated overexpression of PKC zeta, or the other atypical PKC member PKC iota/lambda, alters the basal and impairs the insulin-regulated expressions of glucokinase, sterol regulatory element-binding protein 1c, the cytosolic form of phosphoenolpyruvate carboxykinase, the catalytic subunit of glucose 6-phosphatase, and insulin like growth factorbinding protein 1 in ZL primary hepatocytes. PKC zeta or PKC iota/lambda overexpression also reduces the protein level of insulin receptor substrate 1, and the insulin-induced phosphorylation of AKT at Ser473 and Thr308. Additionally, PKC iota/lambda overexpression impairs the insulin-induced Prckz expression, indicating the crosstalk between PKC zeta and PKC iota/lambda. We conclude that the PKC zeta expression is elevated in hepatocytes of insulin resistant ZF rats. Overexpressions of aPKCs in primary hepatocytes impair insulin signal transduction, and in turn, the downstream insulin-regulated gene expression. These data suggest that elevation of aPKC expression may contribute to the hepatic insulin resistance at gene expression level.

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