期刊
PLOS ONE
卷 9, 期 11, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0113020
关键词
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资金
- National Institutes of Health (NIH) [P30DK020593, P30DK058404, U24DK059637]
- NIH [F32DK091181, R01DK085064]
- Vanderbilt University through the Louise B. McGavock Chair
beta-cells in the pancreatic islet respond to elevated plasma glucose by secreting insulin to maintain glucose homeostasis. In addition to glucose stimulation, insulin secretion is modulated by numerous G-protein coupled receptors (GPCRs). The GPCR ligands Kisspeptin-10 (KP) and glucagon-like peptide-1 (GLP-1) potentiate insulin secretion through G(q) and G(s)-coupled receptors, respectively. Despite many studies, the signaling mechanisms by which KP and GLP-1 potentiate insulin release are not thoroughly understood. We investigated the downstream signaling pathways of these ligands and their affects on cellular redox potential, intracellular calcium activity ([Ca2+](i)), and insulin secretion from beta-cells within intact murine islets. In contrast to previous studies performed on single beta-cells, neither KP nor GLP-1 affect [Ca2+] i upon stimulation with glucose. KP significantly increases the cellular redox potential, while no effect is observed with GLP-1, suggesting that KP and GLP-1 potentiate insulin secretion through different mechanisms. Co-treatment with KP and the G(beta gamma)-subunit inhibitor gallein inhibits insulin secretion similar to that observed with gallein alone, while co-treatment with gallein and GLP-1 does not differ from GLP-1 alone. In contrast, co-treatment with the G(beta gamma) activator mSIRK and either KP or GLP-1 stimulates insulin release similar to mSIRK alone. Neither gallein nor mSIRK alter [Ca2+] i activity in the presence of KP or GLP-1. These data suggest that KP likely alters insulin secretion through a G(beta gamma)-dependent process that stimulates glucose metabolism without altering Ca2+ activity, while GLP-1 does so, at least partly, through a G(alpha)-dependent pathway that is independent of both metabolism and Ca2+.
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