期刊
PLOS ONE
卷 9, 期 8, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0104667
关键词
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资金
- National Natural Science Foundation of China [41376135]
- Doctoral Fund of Ministry of Education of China [20133104110006]
- Innovation Program of Shanghai Municipal Education Commission, China [14ZZ144]
- Construction Program of Shanghai Committee of Science and Technology, China [11DZ2280300]
White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41 +/- 0.17 min at 39 degrees C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.
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