期刊
PLOS ONE
卷 9, 期 9, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0108286
关键词
-
资金
- National Nature Science Foundation of China [30973449, 81172163, 81272515, 81272338, 81200706]
- National Key Sci-Tech Special Project of China [2009ZX09103-642, 2013ZX09102053]
- Program for Doctoral Station in University [20120171110053, 20130171110053]
- Key Project of Nature Science Foundation of Guangdong Province, China [10251008901000009]
- Key Sci-tech Research Project of Guangdong Province, China [2011B031200006]
- Guandong Natural Science Fund [S2012010009250, S2012040006986]
- Key Sci-tech Research Project of Guangzhou Municipality, China [2011Y1-00017-8, 12A52061519]
- Program for Young Teacher in University [10YKPY28]
- Changjiang Scholars and Innovative Research Team in University [985, PCSIRT 0947]
G503 is an anthraquinone compound isolated from the secondary metabolites of a mangrove endophytic fungus from the South China Sea. The present study elucidates the anti-tumor activity and the underlying mechanism of G503. Cell viability assay performed in nine cancer cell lines and two normal cell lines demonstrated that the gastric cancer cell line SGC7901 is the most G503-sensitive cancer cells. G503 induced SGC7901 cell death via apoptosis. G503 exposure activated caspases-3, 8 and -9. Pretreatment with the pan-caspase inhibitor Z-VAD-FMK and caspase-9 inhibitor Z-LEHD-FMK, but not caspase-8 inbibitor Z-IETD-FMK, attenuated the effect of G503. These results suggested that the intrinsic mitochondrial apoptosis pathway, rather than the extrinsic pathway, was involved in G503-induced apoptosis. Furthermore, G503 increased the ratio of Bax to Bcl-2 in the mitochondria and decreased the ratio in the cytosol. G503 treatment resulted in mitochondrial depolarization, cytochrome c release and the subsequent cleavage of caspase -9 and -3. Moreover, it is reported that the endoplasmic reticulum apoptosis pathway may also be activated by G503 by inducing capase-4 cleavage. In consideration of the lower 50% inhibitory concentration for gastric cancer cells, G503 may serve as a promising candidate for gastric cancer chemotherapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据