期刊
PLOS ONE
卷 9, 期 6, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0101322
关键词
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资金
- Defense Advanced Research Projects Agency (DARPA) [HR0011-10-0052]
- Defense Threat Reduction Agency (DTRA) [HDTRA1-12-00007]
- Cancer Prevention Research Institute of Texas (CPRIT)
Rabbits have been used extensively as a model system for h elucidation of the mechanism of immunoglobulin diversification and for he production of antibodies. We employed Next Generation Sequencing to analyze Ig germline V and J gene usage, CDR3 length and amino acid composition, and gene conversion frequencies within the functional (transcribed) IgG repertoire of he New Zealand white rabbit (Oryctolagus cuniculus). Several previously unannotated rabbit heavy chain variable (VH) and light chain variable (VL) germline elements were deduced bioinformatically using multidimensional scaling and k-means clustering methods. We estimated the gene conversion frequency in the rabbit at 23% of IgG sequences with a mean gene conversion tract length of 59 +/- 6 bp. Sequencing and gene conversion analysis of the chicken, human, and mouse repertoires revealed that gene conversion occurs much more extensively in the chicken (frequency 70%, tract length 79 +/- 57 bp), was observed to a small, yet statistically significant extent in humans, but was virtually absent in mice
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