期刊
PLOS ONE
卷 9, 期 2, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0088251
关键词
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资金
- British Heart Foundation [CH/08/001]
- Wellcome Trust [80373]
- MRC [G0401026, G0100152]
- EU [215597]
- Medical Research Council [G0100152, G0401026, MR/K015664/1, G1100041] Funding Source: researchfish
- MRC [G0401026, G0100152, MR/K015664/1, G1100041] Funding Source: UKRI
Podosomes are highly dynamic actin-rich adhesive structures formed predominantly by cells of the monocytic lineage, which degrade the extracellular matrix. They consist of a core of F-actin and actin-regulating proteins, surrounded by a ring of adhesion-associated proteins such as vinculin. We have characterised the structure of podosomes in macrophages, particularly the structure of the ring, using three super-resolution fluorescence microscopy techniques: stimulated emission depletion microscopy, structured illumination microscopy and localisation microscopy. Rather than being round, as previously assumed, we found the vinculin ring to be created from relatively straight strands of vinculin, resulting in a distinctly polygonal shape. The strands bind preferentially at angles between 116 degrees and 135 degrees. Furthermore, adjacent vinculin strands are observed nucleating at the corners of the podosomes, suggesting a mechanism for podosome growth.
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