期刊
PLOS ONE
卷 9, 期 2, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0085230
关键词
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资金
- Fondo de Investigacion Sanitaria en Espana (FIS) [INTRASALUD PS09/02029, RD09/0076/00103]
- Fundacion Eugenio Rodriguez Pascual
- Red Espanola de Investigacion en SIDA (RIS) [RETIC RD06/0006/0035, RD12-0017-0037]
- Fundacion para la Investigacion y la Prevencion del Sida en Espana'' (FIPSE)
- Comunidad de Madrid [INDISNET S-2010/BMD-2332]
- Fondo de Investigacion Sanitaria [CA10/01274]
Background: The course of human immunodeficiency virus type-1 (HIV-1) infection is influenced by a complex interplay between viral and host factors. HIV infection stimulates several proinflammatory genes, such as cyclooxigense-2 (COX-2), which leads to an increase in prostaglandin (PG) levels in the plasma of HIV-1-infected patients. These genes play an indeterminate role in HIV replication and pathogenesis. The effect of prostaglandin E-2 (PGE(2)) on HIV infection is quite controversial and even contradictory, so we sought to determine the role of PGE(2) and the signal transduction pathways involved in HIV infection to elucidate possible new targets for antiretrovirals. Results: Our results suggest that PGE(2) post-infection treatment acts in the late stages of the viral cycle to reduce HIV replication. Interestingly, viral protein synthesis was not affected, but a loss of progeny virus production was observed. No modulation of CD4 CXCR4 and CCR5 receptor expression, cell proliferation, or activation after PGE(2) treatment was detected. Moreover, PGE(2) induced an increase in intracellular cAMP (cyclic AMP) levels through the EP2/EP4 receptors. PGE(2) effects were mimicked by dbcAMP and by a specific Epac (exchange protein directly activated by cyclic AMP) agonist, 8-Cpt-cAMP. Treatment with PGE(2) increased Rap1 activity, decreased RhoA activity and subsequently reduced the polymerization of actin by approximately 30% compared with untreated cells. In connection with this finding, polarized viral assembly platforms enriched in Gag were disrupted, altering HIV cell-to-cell transfer and the infectivity of new virions. Conclusions: Our results demonstrate that PGE(2), through Epac and Rap activation, alters the transport of newly synthesized HIV-1 components to the assembly site, reducing the release and infectivity of new cell-free virions and cell-to-cell HIV-1 transfer.
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