期刊
PLOS ONE
卷 9, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0086982
关键词
-
资金
- National Natural Science Fund [30971766]
- Hebei Provincial Natural Science Fund [C2009000278]
- Doctor Fund of Hebei University of Science and Technology [QD201046]
Genes for V-H+-ATPase subunits were identified and cloned from the salt-tolerant wheat mutant RH8706-49. Sequences of these genes are highly conserved in plants. Overexpression of these genes in Arabidopsis thaliana improved its salt tolerance, and increased the activities of V-H+-ATPase and Na+/H+ exchange, with the largest increase in plants carrying the c subunit of V-H+-ATPase. Results from quantitative RT-PCR analysis indicated that the mRNA level of each V-H+-ATPase subunit in the Arabidopsis increased under salt stress. Overall, our results suggest that each V-H+-ATPase subunit plays a key role in enhancing salt tolerance in plants.
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