期刊
PLOS ONE
卷 9, 期 2, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0089883
关键词
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资金
- USDA CSREES Hatch [WIS01195]
- Intelligent Synthetic Biology Center of Global Frontier Project [2011-0031955]
- Ministry of Education, Science and Technology
- Basic Science Research Program through the National Research Foundation (NRF) of Korea
- Ministry of Education [NRF 2012R1A1A4A01012864]
- Ministry of Education, Science and Technology, Republic of Korea
Fungal development and secondary metabolism is intimately associated via activities of the fungi-specific velvet family proteins including VeA, VosA, VelB and VelC. Among these, VelC has not been characterized in Aspergillus nidulans. In this study, we characterize the role of VelC in asexual and sexual development in A. nidulans. The velC mRNA specifically accumulates during the early phase of sexual development. The deletion of velC leads to increased number of conidia and reduced production of sexual fruiting bodies (cleistothecia). In the velC deletion mutant, mRNA levels of the brlA, abaA, wetA and vosA genes that control sequential activation of asexual sporulation increase. Overexpression of velC causes increased formation of cleistothecia. These results suggest that VelC functions as a positive regulator of sexual development. VelC is one of the five proteins that physically interact with VosA in yeast two-hybrid and GST pull down analyses. The Delta velC Delta vosA double mutant produced fewer cleistothecia and behaved similar to the DvosA mutant, suggesting that VosA is epistatic to VelC in sexual development, and that VelC might mediate control of sex through interacting with VosA at specific life stages for sexual fruiting.
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